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Literature summary extracted from

  • Lapeyre, B.; Purushothaman, S.K.
    Spb1p-directed formation of Gm2922 in the ribosome catalytic center occurs at a late processing stage (2004), Mol. Cell, 16, 663-669.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.167 D52A when the mutant protein is the sole source of Spb1p in the cell (strain YBL4637), it leads to a severe growth defect with a generation time of about 300 min. No formation of 2'-O-methylguanosine2922 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.167 S-adenosyl-L-methionine + guanosine2922 in 27S pre-rRNA Saccharomyces cerevisiae Spb1p is a site-specific 2'-O-ribose RNA MTase that catalyzes the formation of Gm2922, a universally conserved position of the catalytic center of the ribosome that is essential for translation. The enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation. 2'-O-methylguanosine2922 appears at a late processing stage, during the maturation of the 27S pre-rRNA S-adenosyl-L-homocysteine + 2'-O-methylguanosine2922 in 27S pre-rRNA
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 ?
2.1.1.167 S-adenosyl-L-methionine + uridine2918 in 25S rRNA Saccharomyces cerevisiae the enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.167 Saccharomyces cerevisiae
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-
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.167 S-adenosyl-L-methionine + guanosine2922 in 27S pre-rRNA Spb1p is a site-specific 2'-O-ribose RNA MTase that catalyzes the formation of Gm2922, a universally conserved position of the catalytic center of the ribosome that is essential for translation. The enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation. 2'-O-methylguanosine2922 appears at a late processing stage, during the maturation of the 27S pre-rRNA Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methylguanosine2922 in 27S pre-rRNA
-
 ?
2.1.1.167 S-adenosyl-L-methionine + uridine2918 in 25S rRNA the enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.167 Spb1p
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 Saccharomyces cerevisiae